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17

Fernando Campos

Evaluación de viabilidad celular en constructos

4.

Fernández-Segura E, Cañizares FJ, Cubero MA, Revelles F,

Campos A.

Electron probe X-ray microanalysis of cultured epithelial tumour cells with scanning electron microscopy.

Cell Biol Int. 1997; 21(10): 665-669.

5.

Garzón I, Perez-Kohler B, Garrido-Gómez J, Carriel V, Nieto-

Aguilar R, Martín-Piedra MA, Garc

ía-Honduvilla N

,

Buján J

,

Campos A.

Alaminos M

. Evaluation of the cell viability of

human Wharton’s jelly stem cells for use in cell therapy. Tissue

Eng. Part C Methods. 2012; 18:408-419.

6.

Martín-Piedra MA, Garzón I, Oliveira AC, Alfonso-Rodríguez

CA, Carriel V, Scionti G. y Alaminos M.

Cell viability and proliferation capability of long-term human dental pulp stem cell cultures.

Cytotherapy. 2014; 16: 266-277.

7.

Salido M, Vilches J, y Roomans GM. Changes in elemental

concentrations in LNCaP cells are associated with a protective

effect of neuropeptides on etoposide-induced apoptosis. Cell

Biol Int. 2004; 28(5): 397-402.

8.

Sánchez-Quevedo MC, Alaminos M, Capitan LM, Moreu

G, Garzón I, Crespo PV, y Campos A. Histological and

histochemical evaluation of human oral mucosa constructs

developed by tissue engineering. Histol Histopathol. 2007;

22(6): 631-640.

9.

Scionti G, Moral M, Toledano M, Osorio R, Durán JD, Alaminos

M, Campos A, López-López MT.

Effect of the hydration on the biomechanical properties in a fibrin-agarose tissue-like model.

J Biomed Mater Res. 2014; 102(8): 2573-2582.

10. San Martin S, Alaminos M, Zorn TM, Sánchez-Quevedo

MC, Garzón I, Rodriguez IA, Campos A.

The effects of fibrin and fibrin-agarose on the extracellular matrix profile of bioengineered oral mucosa.J

Tissue Eng Regen Med. 2013;

7(1): 10-19.

11. Rodríguez IA, López-López MT, Oliveira AC, Sánchez-

Quevedo MC, Campos A, Alaminos M, Durán JD.

Rheological characterization of human fibrin and fibrin-agarose oral mucosa substitutes generated by tissue engineering.

J Tissue

Eng Regen Med. 2012; 6(8): 636-644.

12. Alaminos M, Garzón I, Sánchez-Quevedo MC, Moreu G,

González-Andrades M, Fernández-Montoya A, Campos A.

Time-course study of histological and genetic patterns of differentiation in human engineered oral mucosa.

J Tissue

Eng. Regen Med. 2007; 1(5): 350-359.

13. Garzon I, Serrato D, Roda O, Del Carmen Sanchez-Quevedo M,

Gonzales-Jaranay M, Moreu G, Nieto-Aguilar R, Alaminos M,

Campos A.

In vitro cytokeratin expression profiling of human oral mucosa substitutes developed by tissue engineering.

Int J

Artif Organs. 2009; 32(10): 711-719.

14. Alaminos M, Del Carmen Sánchez-Quevedo M, Muñoz-Avila

JI, Serrano D, Medialdea S, Carreras I, Campos A.

Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold.

Invest Ophthalmol Vis Sci. 2006; 47(8): 3311-3317.

15. Carriel V, Garzón I, Jiménez JM, Oliveira AC, Arias-Santiago S,

Campos A, Sánchez-Quevedo MC, Alaminos M.

Epithelial and stromal developmental patterns in a novel substitute of the human skin generated with fibrin-agarose biomaterials.

Cells

Tissues Organs. 2012; 196(1): 1-12.

16. Warley A.

Development and comparison of the methods for quantitative electron probe X-ray microanalysis analysis of thin specimens and their application to biological material.

J

Microsc. 2016; 261(2): 177-84.

17. Von Euler A, Wroblewski R, Roomans GM. Correction for

extraneous background in X-ray microanalysis of cell cultures.

Scanning Microsc. 1992; 6(2): 451-456.

18. Zierold K, Schäfer D. Preparation of cultured and isolated cells

for X-ray microanalysis. Scanning Microsc. 1988; 2(3):1775-1790.

19. Warley A, Skepper JN. Long freeze-drying times are not

necessary during the preparation of thin sections for X-ray

microanalysis. J Microsc. 2000; 198(Pt 2): 116-123.

20. Sánchez-Quevedo MC, Nieto-Albano OH, García JM, Gómez

de Ferraris ME, Campos A. Electron probe microanalysis of

permanent human enamel and dentine. A methodological

and quantitative study. Histol Histopathol. 1998; 13: 109-113.

21. Statham PJ, Pawley JB. A new method for particle X-ray

microanalysis on peak to background measurements.

Scanning Electron Microsc. 1978; 1: 469-478.

22.

Boekestein A

,

Stols AL,

Stadhouders AM

. Quantitation in x

ray microanalysis of biological bulk specimens.

Scan Electron Microsc .

1980;

(Pt 2): 321-334.

23. Alaminos M, Sánchez-Quevedo MC, Muñoz-Avila JI, García

JM, Crespo PV, González-Andrades M, Campos A. Evaluation

of the viability of cultured corneal endothelial cells by

quantitative electron probe X-ray microanalysis. J Cell Physiol.

2007; 211: 692-698.

24. Rodríguez-Morata A, Garzón I, Alaminos M, Garcia-Honduvilla

N, Sánchez-Quevedo MC, Bujan J, Campos A.

Cell viability and prostacyclin release in cultured human umbilical vein endothelial cells.

Ann Vasc Surg. 2008; 22(3): 440-448.

25. Garzón I, Carriel V, Marín-Fernández AB, Oliveira AC, Garrido-

Gómez J,

Campos A

,

Sánchez-Quevedo MC,

Alaminos M

. A

Combined Approach for the Assessment of Cell Viability and

Cell Functionality of Human Fibrochondrocytes for Use in

Tissue Engineering. PLoS ONE. 2012: 7: e51961.

26. Martín Piedra MA, Garzón I, Sánchez-Quevedo MC, Alaminos

M. Evaluación de la viabilidad celular y patrones apoptóticos

en células madre aisladas de la pulpa dental humana Actual

Med. 2012; 97: 006- 012.

27.

Ahmed TA

,

Dare EV

,

Hincke M

. Fibrin: a versatile scaffold for

tissue engineering

applications.

Tissue Eng Part B Rev.

2008;

14(2): 199-215.

28.

De

la Puente P,

Ludeña D

. Cell culture in autologous fibrin

scaffolds for applications in

tissue engineering

.

Exp Cell Res.

2014; 322(1): 1-11.

29. Mosesson MW, Siebenlist KR, Meh DA. The structure and

biological features of fibrinogen and fibrin. Annals of the New

York Academy of Sciences. 2001; 936: 11-30.

30. Ionescu AM, Alaminos M, de la Cruz Cardona J, de Dios García-

López Durán J, González-Andrades M, Ghinea R, Campos A,

Hita E, del Mar Pérez M.

Investigating a novel nanostructured fibrin-agarose biomaterial for human cornea tissue engineering: rheological properties.

J Mech Behav Biomed

Mater. 2011; 4(8): 1963-1973.

31. Carriel V, Garzón I, Alaminos M, Cornelissen M.

Histological assessment in peripheral nerve tissue engineering.

Neural

Regen Res. 2014; 9(18):1657-1660.

32. Roomans GM. X-ray microanalysis of epithelial cells in culture.

Methods Mol. Biol. 2002; 188: 273-289.

33. Fernández-Segura E, Warley A.

Electron probe X-ray microanalysis for the study of cell physiology.

En Allen T.

Methods Cell Biol. Elsevier: UK; 2008.

34. Fernández-Segura E, Cañizares FJ, Cubero MA, Warley A,

Campos A. Changes in elemental content during apoptotic cell

death studied by electron probe X-ray microanálisis. Exp Cell

Res. 1999; 253: 454-462.